human lung bronchial epithelial cell line beas 2b cells  (ATCC)

Journal: Antiviral research

Article Title: Influenza virus NS1- C/EBPβ gene regulatory complex inhibits RIG-I transcription

doi: 10.1016/j.antiviral.2020.104747

Figure Lengend Snippet: Heat map of differentially expressed genes based on PCR Array analysis. NHBE cells were transfected with vector, hC/EBPβ, control siRNA or hC/EBPβ siRNA for 24h. Cells were then mock infected or infected with PR8 (1.0 MOI) for 6 h. Gene expression were analysed by real-time RT-PCR using PCR array kit as per manufacturer protocol. Data shown are average from two independent experiments. The scale shows the level of gene expression where red corresponds to upregulation (log2 fold).

Article Snippet: Human lung epithelial cell line A549, and normal human bronchial epithelial (NHBE) cells (Lonza, Switzerland) were maintained as described ( Ranjan et al., 2010 ).

Techniques: Transfection, Plasmid Preparation, Control, Infection, Gene Expression, Quantitative RT-PCR

Journal: Respiratory Research

Article Title: Human epididymis protein 4 aggravates airway inflammation and remodeling in chronic obstructive pulmonary disease

doi: 10.1186/s12931-022-02040-7

Figure Lengend Snippet: CSE enhanced HE4 expression in HBE cells but not in HPF cells. A Different concentrations of CSE increased HE4 mRNA expression in HBE cells (n = 3). B, C The image of western blot was shown and the band intensity was quantified by ImageJ (n = 3). Compared with controls, 5% CSE and 10% CSE markedly enhanced HE4 protein expression in HBE cells. D The secretory HE4 levels were detected in collected CSE-exposed HBE supernatants using ELISA (n = 4). E, F CSE had no obvious impact on HE4 expression in HPF cells (n = 3). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05, **P < 0.01, and ***P < 0.001 represent significant differences relative to controls. CSE, cigarette smoke extract; HE4, human epididymis protein 4; HBE, human bronchial epithelial; HPF, human pulmonary fibroblast

Article Snippet: Human lung bronchial epithelial cell line (HBE) was obtained from the Boster Biotech Co., Ltd (Wuhan, China).

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Respiratory Research

Article Title: Human epididymis protein 4 aggravates airway inflammation and remodeling in chronic obstructive pulmonary disease

doi: 10.1186/s12931-022-02040-7

Figure Lengend Snippet: HE4 affected IL-6 release in HBE cells through phosphorylation of NFκB-p65. A–C The transfection of siRNA in HBE cells significantly lowered HE4 mRNA expression (n = 3), secretory level (n = 3) and intracellular protein expression (n = 4). A , B, D Infecting lentivirus expressing HE4 markedly enhanced HE4 mRNA expression (n = 3), secretory level (n = 4) and intracellular protein expression (n = 4). The western blot images were shown and quantified by ImageJ. E, G HE4 knockdown mitigated IL-6 expression while HE4 overexpression augmented IL-6 expression in HBE cells both in mRNA level (n = 3) and secretory protein level (n = 4). F, H Intervening HE4 expression had no significant impact on IL-8 expression, although reducing HE4 expression can alleviate CSE-induced IL-8 elevation at mRNA level (n = 3–4). I, J Knockdown of HE4 expression reduced phosphorylation of NFκB-p65 relative to the expression of p65 (n = 4). K, L Overexpression of HE4 increased phosphorylation of NFκB-p65 (n = 5). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05, **P < 0.01, and ***P < 0.001 represent significant differences. HE4, human epididymis protein 4; si, small interfering RNA; NC, negative control; Lv-GFP, lentivirus with empty vector carrying green fluorescent protein; Lv-HE4, lentivirus expressing HE4; CSE, cigarette smoke extract; HBE, human bronchial epithelial; IL-6, interleukin-6; IL-8, interleukin-8

Article Snippet: Human lung bronchial epithelial cell line (HBE) was obtained from the Boster Biotech Co., Ltd (Wuhan, China).

Techniques: Phospho-proteomics, Transfection, Expressing, Western Blot, Knockdown, Over Expression, Small Interfering RNA, Negative Control, Plasmid Preparation

Journal: Respiratory Research

Article Title: Human epididymis protein 4 aggravates airway inflammation and remodeling in chronic obstructive pulmonary disease

doi: 10.1186/s12931-022-02040-7

Figure Lengend Snippet: Elevated HE4 expression in CSE-exposed HBE cells was mediated by oxidative stress. A Pretreatment of NAC overtly alleviated CSE-induced HE4 increase (n = 3). B Different concentrations of H 2 O 2 promoted HE4 expression (n = 3). C Pretreating NAC significantly mitigated H 2 O 2 -induced HE4 up-regulation (n = 3). Data are expressed as mean ± SEM. P-values were calculated using one-way ANOVA followed by Newman–Keuls test. *P < 0.05 and **P < 0.01 represent significant differences. HE4, human epididymis protein 4; CSE, cigarette smoke extract; HBE, human bronchial epithelial; DMSO, dimethyl sulfoxide; NAC, N-acetylcysteine

Article Snippet: Human lung bronchial epithelial cell line (HBE) was obtained from the Boster Biotech Co., Ltd (Wuhan, China).

Techniques: Expressing